Solution reagent Capturem™

buffer solutionfor mass spectrometryliquid

Capturem Trypsin provides fast, efficient, and complete digestion of proteins at room temperature, in comparison to typical protein digestion workflows for mass spectrometry (MS) analyses that require four-hour or overnight digestion. By contrast, Capturem Trypsin's rapid and simple protocol allows for an uninterrupted proteomic analysis workflow. This product, based on our novel Capturem technology, provides membrane-immobilized trypsin in a mini spin column or a 96-well plate format. Capturem Trypsin completely digests protein samples in just a few minutes, compared to the time-consuming incubations often required for in-solution digestion, and provides better efficiency with fewer autolyzed fragments. In addition, these Capturem spin columns and plates make it easier to avoid the over-digestion commonly seen with in-solution digests. Process up to 80 µg of protein or 25 µg of antibody on each well or column. More effective proteomic analysis Protein digestion using Capturem Trypsin is more effective than in-solution digestion for preparing samples for MS analysis because it eliminates long digestion times, which may lead to oxidation and other protein modifications, as well as increased autolysis, which can make peptide and protein identification more difficult and less reproducible. Fast, easy protocol Capturem Trypsin includes mini spin columns or 96-well plates and an activation buffer. Capturem protein digestion is performed using a fast, easy protocol. Just load 200 µl of activation buffer, spin for 1–2 minutes, and then load your protein sample and spin for another minute to elute peptides that are ready for downstream analysis.