Medium reagent Lenti-X™

for researchlyophilizedprotein

Lenti-X SARS-CoV-2 Packaging Single Shots (Universal) gives you the flexibility to easily produce high-titer lentivirus pseudotyped with any SARS-CoV-2 spike variant desired. Simply clone any desired SARS-CoV-2 spike variant sequence into the provided expression vector, dilute in sterile water with your transfer vector of choice, and add to a tube of single shots. Then, apply the reconstituted transfection mix to packaging cells in a 10-cm dish. For your convenience, three plasmids encoding the truncated SARS-CoV-2 spike variants D614G, B.1.1.7, and B.1.351 are included. Additional SARS-CoV-2 spike variant mutants can be created using the provided spike variant sequences as template along with the supplied pEmpty vector in a simple, one-step In-Fusion Snap Assembly reaction. Lenti-X SARS-CoV-2 Packaging Single Shots (Universal) allow streamlined production of high-titer lentivirus pseudotyped with your SARS-CoV-2 spike protein of interest by providing pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of Lenti-X lentiviral packaging plasmids. Pseudovirus production simply involved diluting a SARS-CoV-2 spike variant expression vector and your transfer vector of choice in water, adding to a tube of single shots, and applying the mixture to 293T cells (e.g., Lenti-X 293T Cells, in a 10-cm dish. Each kit includes 16 tubes of Lenti-X Packaging Single Shots , the pEmpty Vector for cloning and expression of the desired SARS-CoV-2 spike protein, the Lenti-X SARS-CoV-2 Spike