Solution reagent kit One Shot LA PCR™

DNA polymeraseTaq DNA polymerasefor PCR

One Shot LA PCR Mix Ver. 2.0 enables long-range PCR in a convenient premix format. This long-and-accurate PCR enzyme premix is composed of LA Taq DNA Polymerase, LA PCR Buffer II, and dNTPs provided at 2X concentration in 25-µl aliquots predispensed into 0.2-ml PCR tubes. Simply add an equal volume of solution containing DNA template and primers to each tube to obtain a complete and ready-to-amplify reaction. LA Taq is also offered in the following formats: Individual components—LA Taq DNA polymerase supplied with a tube of optimized LA PCR Buffer II (without Mg2+), MgCl2, and dNTPs TaKaRa LA Taq DNA Polymerase Hot-Start Version—antibody-meditated hot-start PCR to increase reaction specificity and reduce background LA PCR Kit Ver. 2.1—includes all reagents plus controls needed for amplification of long DNA templates Use our LA PCR Genome DNA Set as your long range PCR experimental controls. The set includes purified, high molecular weight human and E. coli genomic DNA plus appropriate primers. A 2X PCR master mix in 25 μl-aliquots in 0.2 ml PCR tubes. The master mix contains Takara LA Taq and all reagents needed for PCR reactions. Simply add template and primers to each tube to begin your PCR. Overview Convenient and reliable long-range PCR Longer and more accurate genomic PCR amplifications Increased reproducibility and reduced optimization for long PCR More Information PCR products PCR products generated with LA Taq contain a mixture of 3'-A overhangs and blunt ends, allowing >80% cloning efficiency into T-vectors. However, the cloning efficiency of longer inserts (>5 kb) into T-vectors is quite low.