Enzyme reagent kit PicoPLEX® Gold

for DNA sequencingNGS

The PicoPLEX Gold Single Cell DNA-Seq Kit (PicoPLEX Gold) is designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms. The kit is based on our patented PicoPLEX technology and is optimized for the reproducible detection of copy number variants (CNVs), single nucleotide variants (SNVs), indels, and small structural variants. The PicoPLEX Gold kit outperforms the leading Multiple Displacement Amplification (MDA) technology for SNV detection. Please note that the PicoPLEX Gold Single Cell DNA-Seq Kit has been reconfigured and no longer includes indexes. We offer compatible index kits in plate format (for multiplexing up to 384 samples) or tube format (for multiplexing up to 96 samples). Our indexing kits are described below. PicoPLEX WGA (PicoPLEX) quasi-random priming technology is the international gold standard for whole genome amplification (WGA) for subsequent detection of CNVs in fixed or unfixed single cells. Initially, PicoPLEX chemistry was created for the reproducible detection of aneuploidies and CNVs. To address the need for accurate detection of SNVs, we further optimized the PicoPLEX WGA chemistry using enzymes, primers, and protocols that improve sequencing coverage, uniformity, and accuracy while increasing the resolution for CNVs, SNVs, indels, and other small structural variants while retaining reproducibility. PicoPLEX Gold provides enhanced PicoPLEX WGA chemistry in the context of a simple, four-step protocol to convert fixed or unfixed single cells into NGS libraries in under three hours with minimal hands-on time.