Our DNA ligation kit for long fragments is a powerful tool for cloning long DNA fragments spanning 2 kb to over 10 kb in length. This kit contains an optimized ligase/buffer system that enables ligation of long fragments without the need for extensive techniques or special expertise. It is especially well-suited for BAC library construction. Compared with other systems, the TaKaRa DNA Ligation Kit LONG results in insertion efficiencies up to 50X higher for high-molecular-weight DNA fragments ranging from 2 kb to 18 kb than other kits. In addition, this kit also enables fast and easy ligation of smaller-sized DNA inserts.
Takara's DNA ligation Kit LONG is a powerful tool for cloning DNA fragments over 10 kb in length. The kit contains an optimized ligase/buffer system which enables ligation of long fragments without difficult techniques and special expertise. It is especially well-suited for the construction of BAC libraries.
Overview
Superior ligation efficiency, especially for long DNA fragments
Simple protocol
Long control vector and insert provided
More Information
Applications
Vector construction using long DNA fragments
BAC library construction
Cloning of long cDNA fragments
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.