DNA polymerase reagent kit 6025

DNA ligasefor nucleic acidsfor DNA purification

The DNA Blunting Kit allows the conversion of 3' and 5' overhangs to blunt or flush ends. This conversion is accomplished simultaneously by the 3'→5' exonuclease and 5'→3' polymerase activities of T4 DNA Polymerase. The resulting blunt-ended DNA can then be ligated into a vector using the same optimized buffer system employed in our: DNA Ligation Kit, Mighty Mix; TaKaRa DNA Ligation Kit LONG; and Quick DNA ligation kit products. The reaction can then be used directly in bacterial transformation or in vitro packaging procedures without the need for further DNA purification. The DNA Blunting Kit allows the conversion of 3' and 5' overhangs to blunt ends. This conversion is accomplished by the 3'→5' exonuclease and 5'→3' polymerase activities of T4 DNA Polymerase. The resulting blunt-ended DNA can be ligated efficiently into a vector using the same optimized buffer system used in Takara's DNA Ligation Kits, then used directly in bacterial transformation or in vitro packaging procedures without DNA purification. More Information Applications Quick and easy blunting of DNA fragment ends Product citations Sambrook, J., Fritsch, E. F. & Maniatis, T. in Mol. Cloning A Lab. Manual. Cold Spring Harb. Lab. Press 931–957 (Cold Spring Harbor Laboratory Press, 1989). Additional product information Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.