Enzyme reagent kit 2910A

clinicalphosphate

Micrococcal Nuclease is an endonuclease that preferentially digests single-stranded DNA or RNA, especially at AT- or AU-rich regions. The enzyme will also digest double-stranded DNA or RNA, making it an essential component of chromatin immunoprecipitation (ChIP) assays. Micrococcal Nuclease digests 5'-phosphodiester bonds of DNA and RNA, yielding 3'-phosphate mononucleotides and oligonucleotides. This enzyme requires Ca2+ as a cofactor for its activity and is completely inactivitated by EDTA or EGTA. Micrococcal Nuclease is an endonuclease that preferentially digests single-stranded DNA and RNA, especially at AT or AU rich regions, but will also digest double-stranded DNA or RNA. This enzyme digests 5'-phosphodiester bonds of DNA and RNA, and yields 3'-phosphate mononucleotides and oligonucleotides. It is commonly used for preparation of nucleosomes during epigenetic analysis studies. This enzyme requires Ca2+ and is completely inactivated by EDTA or EGTA. More Information Applications Digestion of nucleotides included in crude cell extract Digestion of chromatin for nucleosome preparation Chromatin immunoprecipitation (ChIP) Supplied buffer 10X Micrococcal Nuclease [200 mM Tris-HCl (pH 8.0), 50 mM NaCl, 25 mM CaCl2] Form 50 mM Tris-HCl (pH 8.0), 10 mM 2-mercaptoethanol, 50 mM NaCl, 50% glycerol