Research test kit Capturem™

cellular biologyserumplasma

Capturem extracellular vesicle (EV) isolation kits are a complete solution for simple isolation of EVs in <30 min. These columns use a lectin-based binding compound to selectively isolate EVs from various biofluids. Ultracentrifugation is slow, damages EVs, and pulls down contaminants, confounding results. Other methods, like precipitation, are inconsistent, with low yield and purity. Our kits yield pure, intact EVs for downstream analyses, advancing diagnostic research and biomarker discovery. The Capturem Extracellular Vesicle Isolation Kit (Mini) provides a complete solution for the simple and rapid isolation of extracellular vesicles, like exosomes, from plasma and other small volumes of biological fluids. The kit includes 20 single-use, disposable pre-clearing columns; 20 single-use, disposable isolation columns; as well as equilibrium, wash, and elution buffers. Extracellular vesicles are isolated using specially designed Capturem spin columns containing a lectin-based binding compound which is not antibody-based. Overview Consistent, rapid isolation of highly pure, concentrated EVs from up to 850 µl (miniprep) of biofluid, in <30 minutes Capturem method is faster, gentler, and provides higher purity than ultracentrifugation with sufficient yield for downstream applications Purified EVs express exosome protein markers, and little to no contaminant proteins such as calnexin or albumin Sufficient EV yield (up to 1010) for downstream RT-qPCR or NGS analysis of extracellular vesicle cargo Purified EVs display classical morphology, as shown by TEM