Solution reagent kit A559-150

laboratoryfor clinical chemistryliquid

Aspartate aminotransferase (AST) is used for the quantitative determination of aspartate aminotransferase (AST) in human serum. The reagents are used in routine examination and monitoring of therapy and relapses. Method: Kinetic Testing Procedure: Manual Wavelength: 340 nm Linearity: 500 U/L at 37°C Expected Values: 8‐33 U/L at 37°C It is strongly suggested that each laboratory establish its own range of expected values. Reagent Deterioration: Discard the reagents if either appears cloudy or contains particulate matter. The working reagent should be discarded if the initial absorbance, read against distilled water at 340 nm, is below 1.100. Limitations of Procedure: Hemolysis must be avoided as the concentration of AST in red cells is roughly 10 times that of serum. Grossly icteric or turbid specimen may require the use of a sample blank. AST values for neonatal patients have not been established with this procedure.