Bilirubin reagent is used for the quantitative determination of direct bilirubin in human serum. For in vitro diagnostic use.
Method:
Colorimetric
Testing Procedure:
Manual
Wavelength:
560 nm
Linearity:
20 mg/dL
Expected Values:
Infant (after one month) & adults: 0-0.5 mg/dL
It is strongly suggested that each laboratory establish its own range of expected values.
Reagent Deterioration:
The reagent should be discarded if: (1) Sodium Nitrate reagent has a yellow
discoloration; (2) Working reagent fails to achieve assigned assay values of fresh control
sera.
Limitations of Procedure:
Sera with values above 20mg/dL must be diluted 1:1 with isotonic saline, reassayed, and
the final result multiplied by two.
In this assay materials which come in contact with specimens should be clean and free of
contamination by heavy metals, detergents and other chemicals.