For the determination of urea nitrogen in human serum.
Number of Tests:
400 tests
8 x 150 mL bottles
Method:
Kinetic
Testing Procedure:
Manual
Wavelength:
340 nm
Linearity:
80 mg/dL
Expected Values:
7-18 mg/dL
It is strongly suggested that each laboratory establish its own range of expected values.
Reagent Deterioration:
The reagent should be discarded if: (1) Turbidity has occurred; (2) Moisture has
penetrated the vial and caking has occurred; (3) The reconstituted reagent has a reagent
blank absorbance less than 1.000 at 340 nm.
Limitations of Procedure:
Anticoagulants such as fluoride, citrate, and EDTA may inhibit urease and should be
avoided. Ammonium ions present in water or other substances may falsely elevate urea
values.