Clinical chemistry reagent kit I592-100

blood sampleliquidiron

Iron/TIBC Reagent Set is used for the quantitative determination of iron and total iron‐binding capacity in human serum. Number of Tests: 100 tests Testing Procedure: Manual Wavelength: 560 nm Expected Values: Iron, Total = 60 ‐ 150 µgdL TIBC = 250‐400 µgdL Iron Saturation = 20 ‐ 55% It is recommended that each laboratory establish its own range of expected values. Linearity: 500 µgdL Reagent Deterioration: Appearances of turbidity, possible mold growth, or crystal formation that will not readily dissolve are signs of reagent deterioration. Failure to obtain accurate results in the assay of control materials may indicate reagent deterioration Limitations of Procedure: Certain drugs and other substances are known to influence circulating iron levels. Iron contained in hemoglobin does not react in this method; therefore, slight hemolysis will not interfere. However, gross hemolysis (pink or red specimens) will contribute to the absorbance measured at the wavelength used and should be avoided. To make tubes, pipettes, etc. iron free, they must be washed with hot dilute 1:3 hydrochloric or nitric acid, followed by several rinsing with iron‐free deionized or distilled water.