For the quantitative determination of triglycerides in serum or plasma.
Number of Tests:
400 tests
8 x 50 mL
Testing Procedure:
Manual
Reconstituted Stability:
30 days at 2 – 8°C
7 days at 15 ‐ 30°C
Wavelength:
520 nm
Expected Values:
36 – 165 mg/dL
It is recommended that each laboratory establish its own range of expected values.
Linearity:
1000 mg/dL
Reagent Deterioration:
The reagent should be discarded if: (1) The dry powder appears moist and has a dark
discoloration; (2) The reagent fails to meet linearity claims or fails to recover stated
values; (3) The reconstituted reagent has an absorbance of 0.5 or greater against water
at 520 nm. Note: A yellow or pink coloration is normal.
Limitations of Procedure:
Glycerol in rubber stoppers or in contaminated glassware will elevate triglyceride levels.
Lipemic or grossly icteric samples will cause falsely elevated results consequently a
patient blank should be run. Samples with gross hemolysis or high bilirubin values will
produce falsely elevated triglyceride values. A number of drugs and substances affect the
measurement of triglyceride.