Inflammatory bowel disease assay kit ScA096

IgAserumplasma

The immunoenzymatic assay is intended for the diagnosis of IBD using IgA antibodies in human serum or plasma in the general population. The semi-quantitative and quantitative manual assay is designed for professional use in a laboratory. Description: • Microtitre wells are coated with high purified antigen mannan isolated from Saccharomyces cerevisiae. • If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete). • The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells. Advantages: • The total assay time is about 2 hours. • High sensitivity and specificity of the test. • Kit includes CUT-OFF, Positive Control, Negative Control and Calibrators (5, 20, 80, 320　AU/ml). • Semi-quantitative evaluation in the Index of Positivity (IP) or quantitative evaluation in AU/ml. • Ready-to-use, color-coded components. • Single-component substrate. • Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate). Application: • Laboratory test for differential diagnostics of inflammatory bowel disease. Brief assay procedure: 1. Dilute samples (1:51). 2. Incubation at 37 °C for 30 min. 3. Pipette Controls and diluted Samples. 4. Incubate at 37°C for 15 min. 5. Aspirate and wash the wells 5× 6. Pipette Conjugate. 7. Incubate at 37°C for 15 min. 8. Aspirate and wash the wells 5× 9. Pipette Substrate (TMB-Complete). 10. Incubate at 37°C for 30 min.