Serology assay kit YGMA048

infectionYersinia enterocoliticaserum

The Microblot-Array assay is intended for the diagnosis of Yersinia enterocolitica infection using IgG antibodies in human serum or plasma in the general population. The qualitative, semi-quantitative and quantitative automated assay is designed for professional use in a laboratory. Description: • Used Antigen: combination of selected parts of the specific antigens of Yersinia enterocolitica (YopB, YopD, YopM, YopN, LcrV, Ail, Invasin, YscM-Y.Ent). • Recombinant antigens are applied in the form of microdots (spots) in triplets to a nitrocellulose membrane in a microtiter well format. • If specific antibodies are present in the sample, they will bind to the respective antigens. • The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT). • The kit enables 48 tests. • Short incubation periods, total assay time: approximately 1.5 h. • Each individual test includes control spots, which serves to check functionality and sensitivity of the sets, and also the spots for checking the presence of the conjugate. • Positive controls included enabling validation of the tests. • For quantitative evaluation the wells contain calibration spots. • All reagents supplied are ready to use. • The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities). • Evaluation using Microblot-Array reader and the appropriate software. Applications: • Diagnostics of Yersinia infection. • Confirmatory method for ELISA.