EmerTher Magnetic Oral Swab and Saliva DNA Extraction Kit is used to extract DNA from oral swab and saliva.
The kit contains superparamagnetic nanoparticles which are bound to nucleic acids and an efficient extraction system. The magnetic beads are coated through a unique process, enabling strong binding with nucleic acids and easy elution.
The experimental procedure is simple and efficient: 1) ) add oral swab sample (with pre-treatment) or saliva (no pre-treatment) to a lysis-binding buffer to enable DNA release and DNA binding to magnetic beads in one step; 2) apply magnetic force enabling easy wash of the beads with buffers; 3) elute DNA from the beads using an elution solution. The extraction procedure is fully compatible with automation.
Purified DNA can be directly used in a variety of downstream experiments, including PCR, gene sequencing, etc.
Features
High-throughput: fast purification, complete the purification process within 40 min
Automation: compatible with a variety of automatic magnetic bead processors. Pre-filled 96-well plates with necessary solutions are available
High extraction efficiency and high purity: extract 2~11 μg DNA per oral swab, OD260/280 ratios are 1.7-2.0