Highly efficient purification of high quality genomic DNA from 100 μl-1 ml blood
Description
The kit adopts magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality genomic DNA from 100 μl-1 ml of blood. Unique embedded magnetic beads have strong affinity for nucleic acid under certain conditions. When the conditions change, the magnetic beads release adsorbed nucleic acid, thus achieving the purpose of fast separation and purification of nucleic acid. The whole process is safe and convenient. With large extracted genomic DNA fragments, high purity and reliable quality, it is especially suitable for automatic extraction of high-throughput workstations. The DNA purified by the kit can be suitable for various conventional operations, including enzyme digestion, PCR, real time PCR, library construction, Southern blot, chip detection, high throughput sequencing and other experiments.
Features
■ Simple and fast: Ultra-pure genomic DNA can be obtained within 1 hour.
■ High Throughput: It can integrate the automated instrument of pipetting and magnetic rod to carry out high throughput extraction experiments.
■ Safe and non-toxic: No toxic organic reagents such as phenol/ chloroform are needed.
■ High purity: The obtained DNA has high purity and can be directly used in chip detection, high-throughput sequencing and other experiments.
Required Instruments
Magnetic stand, or automated pipetting instruments such as Hamilton Microlab STAR, Beckman Coulter Biomek FX and Capitalbio LabKeeper, or automated magnetic rod instruments such as Thermo KingFisher Flex and Taco.
Required Reagents
Isopropanol, ethanol