Ampicillin reagent kit pEASY®-Blunt E1

for microbiologyfor DNA sequencingprotein

pEASY®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa. • 5 minutes fast ligation of Pfu-amplified PCR products. • Ampicillin resistance. • T7 promoter primer and T7 terminator primer for sequencing. • Bacteriophage T7lac promoter for high level expression. • N-terminal 6×His tag for easy purification. • Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. • E1 Expression Plasmid included as negative control. Shipping Dry ice (-70℃).