Enzyme reagent FlyCut® XhoI
genome editingfor molecular biology

enzyme reagent
enzyme reagent
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Characteristics

Type
enzyme
Applications
for molecular biology, genome editing
Storage temperature

-20 °C
(-4 °F)

Description

FlyCut® XhoI is expressed and purified from E.coli that carries the recombinant XhoI gene. The molecular weight is 27.9 kDa, with the recognition site at C^TCGAG. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation. Recognition Site 5’...C↓TCGAG...3’ 3’...GAGCT↑C...5’ Applications Genomic DNA, plasmid DNA, PCR product Shipping Dry ice (-70 ℃)

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