FlyCut® XhoI is expressed and purified from E.coli that carries the recombinant XhoI gene. The molecular weight is 27.9 kDa, with the recognition site at C^TCGAG. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
Recognition Site
5’...C↓TCGAG...3’
3’...GAGCT↑C...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Shipping
Dry ice (-70 ℃)