Enzyme reagent FlyCut® XbaI
for molecular biologyEscherichia coli

enzyme reagent
enzyme reagent
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Characteristics

Type
enzyme
Applications
for molecular biology
Micro-organism
Escherichia coli
Storage temperature

-20 °C
(-4 °F)

Description

FlyCut® XbaI is expressed and purified from E.coli that carries the recombinant XbaI gene. The molecular weight is 24.7 kDa, with the recognition site at T^CTAGA. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dcm or mammalian CpG methylation, but sensitive to dam methylation. Recognition Site 5’...T↓CTAGA...3’ 3’...AGATC↑T...5’ Applications Genomic DNA, plasmid DNA, PCR product Shipping Dry ice (-70 ℃)

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