FlyCut® XbaI is expressed and purified from E.coli that carries the recombinant XbaI gene. The molecular weight is 24.7 kDa, with the recognition site at T^CTAGA. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dcm or mammalian CpG methylation, but sensitive to dam methylation.
Recognition Site
5’...T↓CTAGA...3’
3’...AGATC↑T...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Shipping
Dry ice (-70 ℃)