- Laboratory
- Laboratory medicine
- Enzyme reagent
- TransGen Biotech Co.
Enzyme reagent FlyCut® SphIfor molecular biologyEscherichia coli
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Characteristics
- Type
- enzyme
- Applications
- for molecular biology
- Micro-organism
- Escherichia coli
- Storage temperature
-20 °C
(-4 °F)
Description
FlyCut® SphI is expressed and purified from E.coli that carries the recombinant SphI gene. The molecular weight is 26.8 kDa, with the recognition site at GCATG^C. The reaction is conducted for 5-10 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...GCATG↓C...3’
3’...C↑GTACG...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Shipping
Dry ice (-70 ℃)
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Restriction/Modification Enzymes
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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.