TransNGS® DNA Library Prep Kit for Illumina® contains buffers and enzymes to generate next generation sequencing libraries for Illumina® SE or PE sequencing platform from 1 ng to1 μg fragmented double-stranded DNA. This kit is suitable for fragmented genome DNA, amplification DNA products, ChIP DNA, or FFPE DNA.
• User-friendly and fast with minimal hands-on time.
• High efficiency with minimal amount of input DNA.
Shipping
Dry ice (-70℃).
Product Contents
Component -
End-repair and A-tailing Reaction Mix - 60 μl - 240 μl - 960 μl
End-repair and A-tailing Enzyme Mix - 30 μl - 120 μl - 480 μl
End-repair and A-tailing Enhancer - 18 μl - 72 μl - 288 μl
TransNGS® Adapter for Illumina® (16 μM) - 30 μl - 120 μl - 480 μl
Adapter Dilution Buffer - 300 μl - 1.2 ml - 5 ml
Adapter-ligation Buffer - 180 μl - 720 μl - 4×720 μl
Adapter-ligation Enzyme - 30 μl - 120 μl - 480 μl
TransNGS® Library Amplification SuperMix (2×) - 150 μl - 600 μl - 4×600 μl
Library Elution Buffer - 1 ml - 4 ml - 4×4 ml
Nuclease-free Water - 1 ml - 1 ml - 5 ml