Indirect calorimeter CaloSys

differential scanningfor preclinical research

The TSE Systems Indirect Gas Calorimetry module measures the animal’s oxygen consumption and carbon dioxide production to calculate key metabolic parameters, including the respiratory exchange rate (RER), and energy expenditure (EE). Specialized isotope sensors such as for 13C can be used for substrate utilization (fat vs. carbohydrate catabolism). The optional new ClimateBoard module adds continuous temperature and humidity environment monitoring to the home cage. This allows for accurate animal welfare monitoring and future refinement in co-housed animal studies and moving to a “direct calorimetry” application. And, in combination with the new optional InfraMot module can be extended to include continuous movement monitoring. Phenomaster offers several versions of gas sensor configurations that differ in time resolution and speed of measurement. In the economical high-speed Multiplex mode where several cages share a sensor pair for O2 and CO2, we use a combination of a new (MOPO) magneto-optical paramagnetic oxygen sensor with a T90 of 1.3 seconds and a new (NDIR) optimized optical near-infrared CO2 photometer detector with a T90 of 2.5 seconds. Both are nearly drift-free (less than 1% per week of sensor range) and maintenance-free. The NDIR can be optionally further configured to simultaneously also provide 13C isotope and CH4 methane data. Together this combination can detect changes in O2 and CO2 in the low ppm range at flow rates between 0.34 to 100 L/minute with a measurement time of 15 seconds per cage to reach 99% of the final signal. Importantly this measurement speed does not depend on the number of cages in the system.