Automatic protein analyzer Uncle

fluorescencelight scatteringlaboratory

Cracking stability using a pile of one-trick, protein-hungry tools is a ton of work. Uncle combines 3 different measurement modes — fluorescence, Static Light Scattering (SLS) and Dynamic Light Scattering (DLS). So you can crank out all your data in just a few hours, and use way less protein. All the info you’ll get makes picking the best formulation, protein, or viral vector a piece of cake. Get brochure. Tm & T agg Tm with SYPRO (DSF) Isothermal stability Sizing & polydispersity Sizing with thermal ramp Thermal recovery Viscosity kD B22 G22 ΔG Viral capsid stability Uncover way more in one shot Trying new formulations or constructs? Get answers for up to 48 samples in under 2 hours. Measure Tm and Tagg at the same time and know when unfolding leads to aggregation. Add a DLS read before the temp ramp to know if you’ve got aggregate trouble right out of the gate. AAV capsid stability Uncle teams up with SYBR Gold to get a read on when your DNA starts to leak — way before the AAV capsid pops. Quantify initial free DNA and the amount that’s on the loose after a thermal ramp. Know when aggregation gets out of hand and tackle particle titer with SLS and DLS. B22 & kD Get B22 and kD at the same time in the same Uni. Or, switch over to G22 if you’re working at super high concentrations. Learn on the spot if your protein-formulation combo is good to go or risky for aggregation with software that makes things easy to figure out. For super high protein concentrations, G22 is ready to go.