Enzyme reagent Phanta Max

DNA polymerasemonoclonal antibodyfor PCR

Phanta Max Super-Fidelity DNA Polymerase is a new generation superior enzyme based on Phanta DNA Polymerase. Compared with the previous generation, Phanta Max has added the unique elongation factor, specificity-enhancing factor and plateau phase anti-inhibitor factor, which greatly improves the long-fragment amplification ability, amplification specificity and amplification yield. Phanta Max can efficiently amplify up to 40 kb simple templates (e.g. λDNA, plasmids), 20 kb complex templates (e.g. genomic DNA) and 10 kb cDNA. The amplification error rate of Phanta Max is 128-fold lower than that of conventional Taq DNA Polymerase and 6-fold lower than that of Pfu DNA Polymerase. In addition, Phanta Max has a good resistance to PCR inhibitors and can be used for direct PCR amplification of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Phanta Max contains two monoclonal antibodies inhibiting the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. Amplification products are blunt-ended, which are compatible with ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601). Product Advantages The fidelity is 128 times that of Taq DNA Polymerase; The amplification speed is 4-150 times that of conventional polymerase; The optimized buffer system is widely applicable to various templates; Ready-to-use premix, minimizing experimental steps