Buffer solution reagent C311 series
DNA ligasefor PCRphosphate

Buffer solution reagent - C311 series - Vazyme Medical Co.,Ltd - DNA ligase / for PCR / phosphate
Buffer solution reagent - C311 series - Vazyme Medical Co.,Ltd - DNA ligase / for PCR / phosphate
Buffer solution reagent - C311 series - Vazyme Medical Co.,Ltd - DNA ligase / for PCR / phosphate - image - 2
Buffer solution reagent - C311 series - Vazyme Medical Co.,Ltd - DNA ligase / for PCR / phosphate - image - 3
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Characteristics

Type
buffer solution, DNA ligase
Applications
for PCR
Tested parameter
phosphate
Storage temperature

-30 °C, -15 °C
(-22 °F, 5 °F)

Description

5minTM Universal Ligation Mix is ​​a ready-to-use 2× premix that mixes enzyme and Buffer. It contains T4 DNA ligase, which catalyzes the formation of adjacent 5´-phosphate ends and 3´-hydroxyl ends on double-stranded DNA or RNA to form phosphate diphosphates. Ester bond. This kit is not only suitable for sticky end ligation, but also compatible with DNA ligation with blunt ends and single-base overhanging ends. The optimized connection enhancer and reaction buffer make the reaction more efficient and convenient. The reaction can be quickly connected at 25°C for 5 minutes. The ligation product can directly transform a variety of chemically competent cells. Product Advantages Compatible: compatible with sticky ends, smooth ends, TA connection, Linker or Adapter connection; Fast: 25℃, 5 minutes can be connected quickly; High efficiency: High cloning efficiency.
*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.