Chromatography reagent HQ030307001E
resinfor protein purificationglutathione

chromatography reagent
chromatography reagent
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Characteristics

Type
chromatography, resin
Applications
for protein purification
Tested parameter
glutathione
Storage temperature

Max.: 30 °C
(86 °F)

Min.: 4 °C
(39 °F)

Description

GST Focurose 4FF is suitable for the separation and purification of GST-tagged proteins, glutathione S-transferase (GST), or glutathione-dependent proteins. Prepacked columns require the use of a chromatography system, peristaltic pump, or syringe. Features Fast and simple (one-step purification) High capacity, fast flow rate, and easy scale-up Gentle elution conditions that preserve the biological activity of the protein Performance Parameters Highly cross-linked 4% agarose Particle size range 45-165µm Average particle size (D50) 90±5µm Binding capacity ≥20 mg (GST)/mL Chemical stability All commonly used aqueous solutions, such as: 1M acetate buffer, pH 4.0, 0.1M NaOH, 70% ethanol, 8M urea, 6M guanidine hydrochloride. Linear flow velocity (0.3 MPa) ≥250 cm/h Operating pressure ≤0.3MPa Storage solution 20% ethanol

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