GST Focurose 4FF is suitable for the separation and purification of GST-tagged proteins, glutathione S-transferase (GST), or glutathione-dependent proteins. Prepacked columns require the use of a chromatography system, peristaltic pump, or syringe.
Features
Fast and simple (one-step purification)
High capacity, fast flow rate, and easy scale-up
Gentle elution conditions that preserve the biological activity of the protein
Performance Parameters
Highly cross-linked 4% agarose
Particle size range
45-165µm
Average particle size (D50)
90±5µm
Binding capacity
≥20 mg (GST)/mL
Chemical stability
All commonly used aqueous solutions, such as: 1M acetate buffer, pH 4.0, 0.1M NaOH, 70% ethanol, 8M urea, 6M guanidine hydrochloride.
Linear flow velocity (0.3 MPa)
≥250 cm/h
Operating pressure
≤0.3MPa
Storage solution
20% ethanol