Chromatography reagent HQ060312 series

resinfor protein purification

Ni Focurose FF(IMAC) utilizes the interaction between Ni2+ and certain amino acids on the side chains of proteins (primarily histidine, cysteine, and tryptophan) for separation and purification. It is suitable for the separation and purification of His-tagged proteins and other biomolecules that interact with Ni2+. Features Fast and simple (one-step purification) Wide applicability and easy operation, suitable for gravity columns and prepacked columns (peristaltic pump or chromatography systems) Multiple choices, can chelate various metal ions (such as Cu2+, Zn2+, Fe2+, Co2+, Ca2+, etc.) for use Compared to Ni Focurose FF (IDA), Ni2+ detachment is low and it has broad reagent compatibility Note: When chelating Ca2+, avoid using phosphate buffer (as it may form precipitates) Performance Parameters Highly cross-linked 6% agarose Particle size range 45-165µm Average particle size (D50) 90±5µm Binding capacity ≥40 mg (His-tagged protein)/mL (resin) Metal ion loading 16-23 μmol (Ni2+) / mL (resin) pH stability 3-12 (long-term) 2-14 (short-term) Chemical stability Avoid using chelating agents (such as EDTA, EGTA) and reducing agents (such as DTT and DTE) in all commonly used aqueous solutions and buffers. Linear flow velocity (0.3 MPa) ≥300 cm/h Operating pressure ≤0.3MPa Storage solution 20% ethanol