Resin reagent HQ060313 series

for protein purification

Ni Focurose FF (TED) is a purification method that utilizes the interaction between Ni2+ and certain amino acids (primarily histidine, cysteine, and tryptophan) on the side chains of proteins for separation and purification. It is suitable for the purification of His-tagged proteins and other biomolecules that interact with Ni2+. The strong chelating property of Ni2+ allows direct application in the purification of His-tagged proteins expressed in eukaryotic secretion systems, tolerating higher levels of reducing agents and chelators without the need for sample pretreatment. The resin can be easily cleaned and regenerated without nickel stripping, enabling direct NaOH cleaning. Features Fast and simple (one-step purification) Tolerates higher levels of reducing agents and chelators, allowing direct application of His-tagged proteins expressed in eukaryotic secretion systems without pretreatment, maximizing protein activity preservation. No need for nickel stripping, allowing direct NaOH cleaning, significantly shortening the cleaning cycle. Lower nickel leaching compared to traditional Ni Focurose FF (IDA) and Ni FocuroseFF (IMAC), eliminating the need for repetitive regeneration. Performance Parameters Highly cross-linked 6% agarose Particle size range 45-165µm Average particle size (D50) 90±5µm Binding capacity ≥10 mg (His-tagged protein)/mL (resin) pH stability 3-12 (long-term) 2-14 (short-term) Chemical stability 0.01M HCl, 0.01M NaOH (one week) 20mM EDTA, 10mM DTT, 1M NaOH, 8M urea, 6M HCl guanidine (24 hours) 100mM EDTA, 0.5M imidazole (2 hours) 30% isopropanol (20 minutes) Linear flow velocity (0.3 MPa) ≥300 cm/h