Chromatography reagent HS060302 series
resinfor molecular biology

chromatography reagent
chromatography reagent
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Characteristics

Type
chromatography, resin
Applications
for molecular biology
Storage temperature

Max.: 30 °C
(86 °F)

Min.: 4 °C
(39 °F)

Description

Phenyl Focurose FF(HS) is a hydrophobic ligand coupled to provide interactions with hydrophobic groups on the surface of proteins or antibodies, particularly under high ionic strength conditions (where increased ionic strength enhances the interaction between ligands and hydrophobic groups). This enables the separation and purification of biomolecules. Phenyl Focurose FF(HS) is primarily used for initial sample capture and moderate purification. Features Fast and simple (one-step purification) Compared to reversed-phase chromatography, the ligand concentration on the hydrophobic interaction resin is low, leading to mild elution conditions that help preserve the biological activity of molecules Versatile application: it can be used for initial capture and moderate purification individually, or in combination with ion exchange resins High loading capacity Performance Parameters Highly cross-linked 6% agarose Particle size range 45-165µm Average particle size (D50) 90±5µm pH stability 2-14 (long-term) 3-13 (short-term) Chemical stability Compatible with all common buffer solutions, 1M acetic acid, 1M sodium hydroxide, 8M urea, 6M guanidine hydrochloride, 30% isopropanol, and 70% ethanol Linear flow velocity (0.3 MPa) ≥300 cm/h Maximum pressure ≤0.3MPa Storage solution 20% ethanol

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