Phenyl Focurose FF(LS) is a hydrophobic ligand coupled to interact with hydrophobic groups on the surface of proteins or antibodies, particularly under high ionic strength conditions (where increased ionic strength enhances the interaction between ligands and hydrophobic groups). This enables the separation and purification of biomolecules. Phenyl Focurose FF(LS) is primarily used for initial sample capture and moderate purification.
Features
Fast and simple (one-step purification)
Compared to reversed-phase chromatography, the ligand concentration on the hydrophobic interaction resin is low, leading to mild elution conditions that help preserve the biological activity of molecules
Versatile application: it can be used for initial capture and moderate purification individually, or in combination with ion exchange resins
High loading capacity
Performance Parameters
Highly cross-linked 6% agarose
Particle size range
45-165µm
Average particle size (D50)
90±5µm
pH stability
2-14 (long-term) 3-13 (short-term)
Chemical stability
Compatible with all common buffer solutions, 1M acetic acid, 1M sodium hydroxide, 8M urea, 6M guanidine hydrochloride, 30% isopropanol, and 70% ethanol
Linear flow velocity (0.3 MPa)
≥300 cm/h
Maximum pressure
≤0.3MPa
Storage solution
20% ethanol