Solution reagent kit ForenSeq

quality controlgenomic DNA

The ForenSeq mtDNA Control Region Kit offers convenient, efficient library prep for interrogating the control region of the mitochondrial genome (mtGenome) with minimal DNA input and optimum sensitivity. Leveraging proven ForenSeq chemistry, the kit produces a concise data set from the entire control region for direct upload to CODIS. Multiplexing capability boosts operational efficiency without increases to costs or time. The ForenSeq mtDNA Control Region Kit is part of an integrated workflow that sequences libraries on the MiSeq FGx Sequencing System and analyzes data in Universal Analysis Software (UAS). Designed, developed, and manufactured as an end-to-end solution for high performance with forensic samples, extensive technical support backs the entire portfolio. Quality-controlled manufacturing ensures reproducibility with the convenience of all-in-one library prep kits. Key Features Reliable Results The PCR-based assay deploys small amplicons sourced from the latest mitochondrial (mtDNA) databases for enhanced variant detection. A tiled approach overlaps the amplicons to prevent the sequence gaps that can lead to data loss, particularly in degraded samples. Complementing this tiled primer design, an enhanced buffer system delivers exceptional resistance to calcium, humic acid, and other relevant PCR inhibitors. Flexible Workflow Options The protocol includes two normalization methods to tailor library prep for a broad range of input material, from high-quality genomic DNA (gDNA) through low-level and complex samples, while a low input requirement expands possibilities for the most challenging samples.