Solution reagent kit ForenSeq™ mtDNA

for forensicsfor DNA analysis

The ForenSeq mtDNA Whole Genome Kit offers a convenient and cost-effective library prep for sequencing the whole mitochondrial genome (mtGenome) with minimal DNA input and exceptional data recovery. Leveraging proven ForenSeq chemistry, the kit produces expanded data sets that promote population studies or provide additional resolution for severely degraded samples. Moreover, this solution has the lowest list price of any commercial whole genome assay. Multiplexing capability further boosts operational efficiency without increases to costs or time. The ForenSeq mtDNA Whole Genome Kit is part of an integrated workflow that sequences libraries on the MiSeq FGx Sequencing System and analyzes data in Universal Analysis Software (UAS). Designed, developed, and manufactured as an end-to-end solution for high performance with forensic samples, extensive technical support backs the entire portfolio. Quality-controlled reagent manufacturing ensures reproducibility with the convenience of all-in-one kitting. Key Features Reliable Results The targeted PCR-based assay deploys small amplicons sourced from the latest mitochondrial (mtDNA) databases for enhanced variant detection. A tiled primer approach overlaps the amplicons to prevent the sequence gaps that can lead to data loss, particularly in degraded samples. Complementing this tiled primer design, an enhanced buffer system delivers exceptional resistance to calcium, humic acid, and other relevant PCR inhibitors. Flexible Workflow Options The protocol includes two normalization methods to tailor library prep for a broad range of input material,