Flu test kit 70 series

influenza Ainfluenza Bfluorescence

Vitassay qPCR kits are ready to use real-time PCR assays designed to amplify and detect pathogen. Vitassay qPCR Kits contains in each well enzymes, buffer, primer and probes as well as an internal control in a lyophilized format. The kits include a positive and a negative control. Identification of pathogen DNA or RNA occurs by the use of target specific primers ans fluorecent labeled hydrolysis probes which hybridize conserved regions in the genomes of the differentpathogens Real-time PCR kit for the qualitative detection and differentiation of Influenza A (Flu A) and B (Flu B) virus in human samples. Principle of the test Vitassay qPCR Flu A + Flu B test is based on the real-time amplification of specific conserved fragments of the M1 gene of Influenza A and Influenza B virus. The viral RNA extracted is transcribed into cDNA using a specific primer by reverse transcription step followed immediately in the same well by polymerase chain reaction. The virus presence is detected by an increase in observed fluorescence during the reaction upon hydrolysis of the fluorescent probe. The assay is based on 5' exonuclease activity that uses two primers and a fluorogenic hydrolysis probe to detect accumulation of the amplified target sequence during the PCR reaction. When the polymerase begins to extend the primers, the probe is hydrolyzed by its 5'-3' exonuclease activity causing the spatial separation of the fluorophore and the quencher.