Vitassay qPCR Zika+Dengue+Chikungunya test allows the detection and differentiation of Zika, Dengue and/or Chikungunya viruses by real-time RT-PCR in clinical samples. The product is intended for use in the diagnosis of Zika virus, Dengue virus and/or Chikungunya virus infections alongside patient clinical data and other laboratory tests outcomes.
Transport and storage
• The resuspended positive control should be stored at -20ºC. In order to avoid
repeated freeze/thaw cycles, it is recommended to distribute the content in
different aliquots.
• Keep all reagents in the darkness.
Additional equipment and material required
• RNA extraction kit
• Real-time PCR instrument (thermocycler) (Attached I)
• Centrifuge for 1.5 mL tubes
• Vortexer
Micropipettes (1-20 µL, 20-200 µL)
• Filter tips
• Powder-free disposal gloves
Summary
Zika (ZIKV), Dengue (DENV) and Chikungunya (CHIKV) viruses are transmitted by the
sting of the same Aedes genus mosquito.
Besides, Zika virus was isolated in 1947 from a rhesus monkey in the Zika forest in
Uganda. Typically, clinical manifestations of human ZIKV disease are similar to many
others derived of arbovirus infections that occur without serious complications and may
include a self-limiting febrile illness, arthralgia, myalgia, headache and maculopapular
rash. Conjunctivitis, retroorbital eye pain, lymphadenopathy and diarrhoea have also
been reported.