Tropical disease test kit 7041004

geneticWest Nile virusclinical

Vitassay qPCR West Nile Virus allows the qualitative detection of West Nile virus by real-time RT-PCR in clinical samples. The product is intended for use in the diagnosis of West Nile virus infections alongside clinical data of the patient and other laboratory tests outcomes. Transport and storage • The reagents and the test can be shipped and stored at 2-40ºC until expiration date stated in the label. • The resuspended positive control should be stored at -20ºC. In order to avoid repeated freeze/thaw cycles, it is recommended to distribute the content in different aliquots. • Keep all reagents in the darkness. Additional equipment and material required • Real-time PCR instrument (thermocycler) (Attached I) • RNA extraction kit • Centrifuge for 1.5 mL tubes • Vortexer • Micropipettes (1-20 µL, 20-200 µL) • Filter tips • Powder-free disposal gloves Summary West Nile virus (WNV), has become one of the main cause of viral encephalitis. The virus was originally isolated in 1937 in Uganda, and later produced epidemic outbreaks in Asia, Europe, Australia and the USA. It is a member of the flavivirus genus and belongs to the Japanese encephalitis antigenic complex of the family Flaviviridae. Flaviviruses are single-strand positive-sense RNA viruses. Thought there are seven known genetic lineages of WNV, lineages 1 and 2 are responsible for the major epidemics in humans and animals. WNV is maintained in nature in a mosquito-bird-mosquito transmission cycle. Mosquitoes of the genus Culex are generally considered the principal vectors of WNV, in particular Cx. Pipiens, while birds are the reservoir hosts of the virus. Human transmission results mostly from mosquito bites.