Vitassay qPCR Malaria 5 allows the detection and differentiation of Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale, Plasmodium malariae and/or Plasmodium knowlesi by real-time PCR in clinical samples. The product is intended for use in the diagnosis of Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale, Plasmodium malariae and/or Plasmodium knowlesi alongside clinical data of the patient and other laboratory tests outcomes.
Transport and storage
• The reagents and the test can be shipped and stored at 2-40ºC until expiration
date stated in the label.
• The resuspended positive control should be stored at -20ºC. In order to avoid
repeated freeze/thaw cycles, it is recommended to distribute the content in different aliquots.
• Keep all reagents in the darkness.
Additional equipment and material required
• DNA extraction kit
• Real-time PCR instrument (thermocycler) (Attached I)
• Centrifuge for 1.5 mL tubes
Vortex
• Micropipettes (1-20 µL, 20-200 µL)
• Filter tips
• Powder-free disposal gloves
Summary
Malaria is a life-threatening disease caused by parasites that are transmitted to people
through the bites of infected female Anopheles mosquitoes. The mosquitoes that spread
malaria are found in Africa, Central and South America, parts of the Caribbean, Asia,
Eastern Europe, and the South Pacific. Malaria causes high levels of morbidity and
mortality in human beings worldwide. In 2018, there were an estimated 228 million
cases of malaria worldwide. The estimated number of malaria deaths stood at 405 000
in 2018. There is no malaria vaccine.