Infection test kit 7041008

DNA extractionClostridium difficileclinical

Vitassay qPCR Clostridium difficile allow the detection and differentiation of Clostridium difficile by real-time PCR in clinical samples. The product is intended for use in the diagnosis of Clostridium difficile infections alongside clinical data of the patient and other laboratory tests outcomes. Transport and storage • The reagents and the test can be shipped and stored at 2-40ºC until expiration date stated in the label. • The resuspended positive control should be stored at -20ºC. In order to avoid repeated freeze/thaw cycles, it is recommended to distribute the content in different aliquots. • Keep all reagents in the darkness. Additional equipment and material required • DNA extraction kit • Real-time PCR instrument (thermocycler) (Attached I) • Centrifuge for 1.5 ml tubes • Vortex • Micropipettes (1-20 µl, 20-200 µl) • Filter tips • Powder-free disposal gloves Summary Clostridium difficile, which belongs to Clostridiaceae and more concretely to the Clostridium genus, is a threat for patients undergoing antibiotic treatment, as it exploits the weakening of the bacterial flora located in the gut, either by colonizing it or overgrowing in it. Nosocomial infections are widely regarded as the main way of contracting pseudomembranous colitis, which is the disease generated by the action of Clostridium difficile toxins. The most common symptom is bloody or watery diarrhea; rarer ones vary from fever, to abdominal pain, changes in bowel habit or leukocytosis.