Infection test kit 7041047

for BK virusfor JC virusclinical

Vitassay qPCR BK & JC allows the detection and differentiation of BK and JC Virus by real-time PCR in clinical samples. The product is intended for use in the diagnosis of BK and/or JC Virus infections alongside clinical data of the patient and other laboratory tests outcomes. Transport and storage • The reagents and the test can be shipped and stored at 2-40ºC until expiration date stated in the label. • The resuspended positive control should be stored at -20ºC. In order to avoid repeated freeze/thaw cycles, it is recommended to distribute the content in different aliquots. • Keep all reagents in the darkness. Additional equipment and material required • DNA extraction kit • Real-time PCR instrument (thermocycler) (Attached I) • Centrifuge for 1.5 mL tubes • Vortexer • Micropipettes (1-20 µL, 20-200 µL) • Filter tips • Powder-free disposal gloves Summary Polyomaviruses are ubiquitous, small, non-enveloped DNA, species-specific viruses which belong to the family Papovaviridae. The two most commonly known human polyomaviruses BK virus (BKV) and JC virus (JCV), were firstly described in the 1970s. Human polyomavirus viruses, infect immunocompetent individuals without specific signs or symptoms. They are able to establish subclinical and persistent infections and may reactivate from latency in case of immunosuppression. Primary infection with both BK and JC virus typically occurs in childhood, with 50% of children having seroconversion for BK virus by 3-4 years of age, and for JC virus by 10- 15 years of age.