Antimicrobial susceptibility test kit 7041057

bacteriafor carbapenemase-producing enterobacteriaceaeclinical

Vitassay qPCR Carbapenemase-generating Enterobacteriaceae allows the qualitative detection and differentiation of the main carbapenemase-encoding genes (NDM, VIM, OXA, KPC and/or IMP) by real-time PCR directly from rectal swabs and from bacterial isolates of clinical samples. The product is intended for use in the diagnosis of carbapenem-resistant Enterobacteriaceae infections alongside the patient’s clinical data and other laboratory tests outcomes. Transport and storage • The reagents and the test can be shipped and stored at 2-40ºC until expiration date stated in the label. • The resuspended positive control should be stored at -20ºC. To avoid repeated freeze/thaw cycles, it is recommended to distribute the content in different aliquots. • Keep all reagents in the darkness. Additional equipment and material required • Collection and transport system. • DNA extraction kit • Real-time PCR instrument (thermocycler) (Attached I) • Centrifuge for 1.5 mL tubes • Laboratory freezers (- 30°C to - 10°C and/or ≤ -70°C). • Vortex • Micropipettes (1-20 µL, 20-200 µL) • Filter tips • Powder-free disposal gloves Summary The emergence and spread of drug-resistant pathogens that have acquired new resistance mechanisms, leading to antimicrobial resistance, continues to threaten our ability to treat common infections. Carbapenems are a potent class of β-lactam antibiotics that are often used as “last-line agents” or “antibiotics of last resort” when infected patients become severely ill or are suspected of harboring resistant bacteria.