Rapid rotavirus test

infectionfor antigensstool

Vitassay Rotavirus is a rapid one step immunochromatographic assay for the qualitative detection of rotavirus in human stool samples. Simple, non-invasive and a highly sensitive screening assay to make a presumptive diagnosis of rotavirus infection. INTRODUCTION Over the last years some viruses have been detected as the cause of diarrhea. Among the viral agents associated with these illnesses, rotavirus is the most important so that their detection is epidemiologically important. Rotavirus infections of adults are usually subclinical but occasionally cause illness in parents of children with rotavirus diarrhea, immunocompromised patients (including those with HIV), the elderly, and travelers to developing countries. In temperate climates, rotavirus diarrhea occurs predominantly during the fall and winter; and in developing countries,seasonality is less marked. PRINCIPLE Vitassay Rotavirus is a qualitative immunochromatographic assay for the detection of rotavirus in human stool samples. The test line zone of the nitrocellulose membrane is pre-coated with monoclonal antibodies against rotavirus. During the process, the sample reacts with the antibodies against rotavirus, forming conjugates. The mixture moves upward on the membrane by capillary action. If the sample is positive, antibodies present on the membrane (test line) capture the conjugate complex and a red line will be visible. Although the sample is positive or negative, the mixture continues to move across the membranes and the green control line always appears.