Vitassay Rotavirus+Adenovirus is a rapid, immunochromatographic, one step assay for the simultaneous qualitative detection of rotavirus and adenovirus in human stool samples. Simple, non-invasive and highly sensitivity immunoassay to make a presumptive diagnosis of rotavirus and/or adenovirus infection
INTRODUCTION
Viral pathogens are the most common cause of gastroenteritis in developed countries. Human rotavirus and adenovirus infections are major causes of acute outbreaks and sporadic cases of gastroenteritis, occurring primarily among children less than 2 years of age. Patient hospitalization is often required, with enormous infection control implications.
PRINCIPLE
Vitassay Rotavirus+Adenovirus is a qualitative immunochromatographic assay for the detection of rotavirus and adenovirus in human stool samples. Strip A: The test line zone of the nitrocellulose membrane is precoated with monoclonal antibodies against rotavirus. Strip B: The test line zone of the nitrocellulose membrane is precoated with monoclonal antibodies against adenovirus. During the process, the sample reacts with the antibodies against rotavirus (strip A) and/or adenovirus (strip B), forming conjugates. The mixture moves upward on the membrane by capillary action. If the sample is rotavirus positive, antibodies present on the membrane (test line) capture the conjugate complex and a red line will be visible in the strip A, and if the sample is adenovirus positive, antibodies present on the membrane (test line) capture the conjugate complex and a red line will be visible in strip B.