Respiratory disease test kit 7041020

for antigensbacteriaBordetella

Real-time PCR kit for the qualitative detection and differentiation of Bordetella pertussis, Bordetella parapertussis y/o Bordetella holmesii in human samples Intended use Vitassay qPCR Bordetella pertussis + B. parapertussis + B. holmesii allows the detection and differentiation of Bordetella pertussis, Bordetella parapertussis y/o Bordetella holmesii by real-time PCR in clinical samples. The product is intended for use in the diagnosis of Bordetella pertussis, Bordetella parapertussis y/o Bordetella holmesii infections alongside clinical data of the patient and other laboratory tests outcomes. Transport and storage • The reagents and the test can be shipped and stored at 2-40ºC until expiration date stated in the label. • The resuspended positive control should be stored at -20ºC. In order to avoid repeated freeze/thaw cycles, it is recommended to distribute the content in different aliquots. • Keep all reagents in the darkness. Additional equipment and material required • DNA extraction kit • Real-time PCR instrument (thermocycler) (Attached I) • Centrifuge for 1.5 mL tubes • Vortexer • Micropipettes (1-20 µL, 20-200 µL) • Filter tips • Powder-free disposal gloves Summary The genus Bordetella bacteria are gram negative bacilli which are isolated from the upper respiratory tract. Bordetella pertussis is the main causative agent of whooping cough, although Bordetella parapertussis, can also produce the disease, but with a milder symptomatology.