Vitassay qPCR Legionella pneumophila allows the detection of Legionella pneumophila by real-time PCR in clinical samples. The product is intended for use in the diagnosis of Legionella pneumophila infections alongside clinical data of the patient and other laboratory tests outcomes.
Transport and storage
• The reagents and the test can be shipped and stored at 2-40ºC until expiration date stated in the label.
• The resuspended positive control should be stored at -20ºC. In order to avoid repeated freeze/thaw cycles, it is recommended to distribute the content in different aliquots.
• Keep all reagents in the darkness.
Additional equipment and material required
• DNA extraction kit
• Real-time PCR instrument (thermocycler) (Attached I)
• Centrifuge for 1.5 mL tubes
• Vortexer
• Micropipettes (1-20 µL, 20-200 µL)
• Filter tips
• Powder-free disposal gloves
Summary
Legionella pneumophila remains a significant cause of morbidity and mortality, also it is considered one of the most common causes of outbreaks of pneumonic illnesses. It belongs to the Legionellaceae family and the Legionella genus. At date, 15 serotypes of L. pneumophila have been identified from which, serotype 1, is confirmed to be responsible for 79% of all culture-confirmed or antigen-confirmed cases. Legionella pneumophila can cause two different illnesses, Pontiac fever, which is the non-pneumonic variety and is characterized by a high attack rate, short incubation period, but zero mortality.. Its symptoms being: fever, chills, dry cough, myalgia, malaise and headache.