Vitassay qPCR Zika allows the qualitative detection of Zika virus by real-time RT-PCR in clinical samples. The product is intended for use in the diagnosis of Zika virus infections alongside clinical data of the patient and other laboratory tests outcomes.
Transport and storage
• The reagents and the test can be shipped and stored at 2-40ºC until expiration date stated in the label.
• The resuspended positive control should be stored at -20ºC. In order to avoid repeated freeze/thaw cycles, it is recommended to distribute the content in different aliquots.
• Keep all reagents in the darkness.
Additional equipment and material required
• Real-time PCR instrument (thermocycler) (Attached I)
• RNA extraction kit
• Centrifuge for 1.5 mL tubes
• Vortexer
• Micropipettes (1-20 µL, 20-200 µL)
• Filter tips
• Powder-free disposal gloves
Summary
Zika virus (ZIKV) is an emerging mosquito-borne pathogen (family Flaviviridae, genus Flavivirus) that was isolated in 1947 from a rhesus monkey in the Zika forest in Uganda. ZIKV is believed to be transmitted to humans by infected Aedes spp. mosquitoes. Several studies have demonstrated that ZIKV is endemic to Africa and Southeast Asia. In fact, in other regions, Zika virus infections have been reported in few cases until 2007 when an epidemic of ZIKV infection in humans occurred in Yap Island and Federated States of Micronesia, in the Pacific region.
In humans, ZIKV infection is characterized by mild fever (37.8°C–38.5°C); arthralgia (notably of small joints of hands and feet); myalgia, headache; retroorbital pain; conjunctivitis; and cutaneous maculopapular rash.