The CHAINFOR HCV qPCR Kits intended for in vitro quantitative diagnosis of HCV nucleic acids from Blood and Plasma. The CHAINFOR HCV qPCR Kits optimized with a Virasens Viral Nucleic Acid Purification Kit and Virasens Viral RNA Purification Kit.
Advantages
– Ready to use
– One tube reaction
– Quantitative Detection
– Exogenous Internal Control
– One-step reaction based experiment procedures
– Accuracy >%95
– Simple, rapid and accurate detection
– High sensitivity detection
Details
The CHAINFOR HCV qPCR Kit targets P gene in the HCV genome based on readout of the FAM channel. The kit also has the internal control based on the readout of the HEX channel. In addition, the kits include highly processive Hot start Taq polymerase enzyme.
The CHAINFOR HCV qPCR Kit was analyzed for cross-reactivity and interference substances, and the results of the analyses showed no cross-reactivity or interference with any of the substances tested.
The CHAINFOR HCV qPCR Kitis compatible with those Real-Time PCR systems: Thermo Scientific Quant Studio 5, BMS Mic, Biorad CFX Connect, Biorad CFX96, Hi-Media.
Polymerase chain reaction (PCR) is a common molecular biology technique used in many disciplines to amplify and detect deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) sequences. In the traditional method, (DNA) cloning and amplification can take days. However, the polymerase chain reaction (PCR) method can reduce this time to hours or even less than an hour.
In addition, the polymerase chain reaction (PCR) is compassionate and requires minimal templates for detecting and amplifying specific sequences.