The CHAINFOR HPV qPCR Kits intended for in vitro qualitative diagnosis of HPV nucleic acids from skin, oral and anogenital samples. The CHAINFOR HPV qPCR Kits a rapid RT-PCR test that enables Real-Time PCR. The CHAINFOR HPV qPCR Kits optimized with a Virasens Genomic DNA Purification Kit From Tissue, viral transport medium (VTFOR) and viral nucleic acid transport medium (VNFOR) .
Advantages
– Ready to use
– 18 targets in one tube reaction
– HPV 16-18 genotyping
– FAM, ROX, Cy5 and HEX channel based detection
– Multiplex reaction based experiment procedures
– Accuracy >%95
– Simple, rapid and accurate detection
– High sensitivity detection
Details
The CHAINFOR HPV qPCR Kit targets P gene in the HPV 16 and 18 genome based on readout of the ROX and Cy5 channels respectively, other 16 HPV variants are detected with FAM channel . The kit also has the internal control based on the readout of the HEX channel. In addition, the kits include highly processive Hot start Taq polymerase enzyme.
The CHAINFOR HPV qPCR Kit was analyzed for cross-reactivity and interference substances, and the results of the analyses showed no cross-reactivity or interference with any of the substances tested.
The CHAINFOR HPV qPCR Kitis compatible with those Real-Time PCR systems: Thermo Scientific Quant Studio 5, BMS Mic, Biorad CFX Connect, Biorad CFX96, Hi-Media.
Polymerase chain reaction (PCR) is a common molecular biology technique used in many disciplines to amplify and detect deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) sequences. In the traditional method, (DNA) cloning and amplification can take days.