Enzyme reagent Tin(exo-)

for isothermal amplification

OptiGene’s novel isothermal mastermix for amplification reactions requiring an initial heat-denaturing step. Suitable for HD-LAMP (Heat-denaturing LAMP). Contains the patented highly thermostable Tin(exo-) LF DNA Polymerase. Extreme thermostability and strong strand-displacement activity enable applications unachievable in a single, closed-tube reaction using alternative isothermal enzymes. Key features: Extreme thermostability* ( < 98ºC ) Strand displacement activity 5’-3’ exonuclease minus 3’-5’ exonuclease minus Advantages of HD-LAMP: Template denaturation step at 95ºC prior to amplification has been shown to increase target sensitivity1 Increased primer specificity – reduces false-priming that may occur at lower temperatures. Simple template extraction (cell-lysis). Sample heat-lysis, amplification and detection in a single tube.