Medical software LD1

for LAMPdesignsharing

Automatic Homology & Template Structure Avoidance Local & Desktop BLAST Options Automatic Sequence Retrieval Sequence View Primer Calculator Data & Database Management Generate Report About LAMP Designer OptiGene offers a software application that is used to design efficient primers for Loop-Mediated Isothermal Amplification, or LAMP, assays. LAMP is an isothermal DNA amplification method invented by the Eiken Chemical Company Ltd of Japan and can be used to amplify a few copies of DNA to 109 copies in as little as 30 minutes. Reverse transcription coupled LAMP can also be applied for amplification of RNA sequences. LAMP employs six specially designed primers that recognise eight distinct regions in the target DNA and the design of these six primers is critical for a successful assay. LAMP Designer retrieves batches of gene sequences directly into the program from on-line sources such as GenBank. The application can perform a BLAST search to avoid cross homologies while designing the primers. It also supports Local and Desktop BLAST options for using custom and unpublished databases. LAMP Designer incorporates a sequence view with primers marked on the sequence, and it calculates a melting temperature, Tm, using nearest neighbour thermodynamic theory and highly accurate SantaLucia values. The software also exports a publication quality report for the designed assays for sharing with colleagues. Data is organised in project format and a local database of sequence information and search results is maintained. Avoid Cross Homologies LAMP Designer automatically interprets BLAST search results