Solution reagent RapiGest SF

sample preparationliquidprotein

Labs around the globe are trying to simplify digestion protocols and improve throughput of analysis. Waters patented RapiGest SF Surfactant was designed with your lab’s efficiency in mind, significantly decreasing proteolytic digestion time, and enabling same-day digestions with mass spectrometry (MS) compatibility. RapiGest SF radically enhances the speed and percent recovery of protein enzymatic digestions. This patented anionic surfactant accelerates the in-solution production of peptides generated by proteases, such as Trypsin, Asp-N, Glu-C, Lys-C, and PNGase F. Many hydrophobic proteins are resistant to proteolysis because their cleavage sites are inaccessible to endoproteases. RapiGest SF facilitates the digestion of proteolytically resistant proteins and increases the solubility of hydrophobic proteins and peptides. RapiGest SF is an MS-compatible mild protein denaturant that helps solubilize and unfold proteins, making them more amenable to cleavage without denaturing or inhibiting common proteolytic enzymes like conventional denaturants. This enables use of lesser amounts of expensive endoproteases. Overview Compatible with liquid chromatography (LC) and MS analysis Enables same-day digestions and analysis Minimal or no post-digestion sample preparation is required Improves efficiency and speed of digestion Simplifies digestion protocols and improves throughput of analysis Does not inhibit enzyme activity, unlike conventional denaturants Allows use of lesser amounts of expensive endoproteases because it is not disruptive of endoprotease activity Recommended Use: For improving protein sequence coverage and in-solution digestion protocols.